PCR-based DNA markers linked to a gall midge resistance gene, Gm4t , has potential for marker-aided selection in rice
Identifieur interne : 003F37 ( Main/Exploration ); précédent : 003F36; suivant : 003F38PCR-based DNA markers linked to a gall midge resistance gene, Gm4t , has potential for marker-aided selection in rice
Auteurs : S. Nair [Inde] ; A. Kumar [Inde] ; M. N. Srivastava [Inde] ; M. Mohan [Inde]Source :
- Theoretical and Applied Genetics [ 0040-5752 ] ; 1996-05-01.
English descriptors
- KwdEn :
- Teeft :
- Abhaya, Amplification, Appl, Biotype, Dna, Fragment, Gall, Gall midge, Gall midge resistance gene, Gene, Genet, Genomic, Genomic dnas, Gin2, Gm4t, Homology, Insect pest, Marker, Midge, Mohan, Molecular markers, Nair, Nematode, Nucleic acids, Orseolia oryzae, Parental dnas, Polymerase, Polymerase chain reaction, Polymorphism, Primer, Proc natl acad, Rapd, Rapd fragment, Rapd fragments, Rapd markers, Rapds, Resistance genes, Resistant, Resistant bulk, Resistant parent, Rflp, Rflp markers, Rice gall midge, Sequence information, Simple sequence, Southern hybridisation, Specific primers, Susceptible, Susceptible bulk, Susceptible parent, Tanksley, Theor, Theor appl genet, Tulsi.
Abstract
Abstract: Rice DNAs from a gall midge resistant variety, ‘Abhaya’, a susceptible variety, ‘Tulsi’ and their F3 progeny were screened using 500 random primers in conjunction with bulked-segregant analysis in a polymerase chain reaction (PCR) with a view to detecting random amplified polymorphic DNAs (RAPDs) linked to the gene, Gm4t, which confers resistance to gall midge, a dipteran insect pest of rice. A total of 454 primers were able to produce a distinct amplification pattern, and 3695 bands/loci were amplified between the phenotypically different parents. Of these, 304 bands were polymorphic between the parents, with 19 being phenotypespecific. One of these primers, E20, amplified 2 bands, E20570 and E20583, which are tightly linked to resistance and susceptibility, respectively. These specific bands were cloned and sequenced, and a 94% sequence homology was found between the two fragments. Two specific 20-mer oligonucleotides were synthesized, based on the sequence information of E20583, for use in PCR amplification directly from genomic DNAs. These PCR primers were able to amplify phenotype-specific bands, a 583-bp fragment in susceptible F3 lines and a 570-bp fragment in resistant F3 lines that had been derived from a cross between the parents, indicating their potential and utility for marker-aided selection of the Gm4t gene in rice. Its use would facilitate the early and efficient selection of resistant genes in plant breeding programmes and even in those areas where the insect is not known to occur. These phenotype-specific bands are single-copy sequences and are being mapped to ascertain their chromosomal location in rice.
Url:
DOI: 10.1007/BF00226086
Affiliations:
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Mohan</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:BDD237F8A59E0215B5325F24C3FA6A0D211E7E0E</idno><date when="1996" year="1996">1996</date><idno type="doi">10.1007/BF00226086</idno><idno type="url">https://api.istex.fr/ark:/67375/1BB-85CDF0XG-X/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000387</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000387</idno><idno type="wicri:Area/Istex/Curation">000387</idno><idno type="wicri:Area/Istex/Checkpoint">001673</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">001673</idno><idno type="wicri:doubleKey">0040-5752:1996:Nair S:pcr:based:dna</idno><idno type="wicri:Area/Main/Merge">003F92</idno><idno type="wicri:Area/Main/Curation">003F37</idno><idno type="wicri:Area/Main/Exploration">003F37</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">PCR-based DNA markers linked to a gall midge resistance gene, Gm4t , has potential for marker-aided selection in rice</title><author><name sortKey="Nair, S" sort="Nair, S" uniqKey="Nair S" first="S." last="Nair">S. Nair</name><affiliation wicri:level="1"><country xml:lang="fr">Inde</country><wicri:regionArea>International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, 110067, New Delhi</wicri:regionArea><wicri:noRegion>New Delhi</wicri:noRegion></affiliation></author><author><name sortKey="Kumar, A" sort="Kumar, A" uniqKey="Kumar A" first="A." last="Kumar">A. Kumar</name><affiliation wicri:level="1"><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Plant Breeding and Genetics, Indira Gandhi Agricultural University, 492012, Raipur</wicri:regionArea><wicri:noRegion>Raipur</wicri:noRegion></affiliation></author><author><name sortKey="Srivastava, M N" sort="Srivastava, M N" uniqKey="Srivastava M" first="M. N." last="Srivastava">M. N. 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Genetics</title><idno type="ISSN">0040-5752</idno><idno type="eISSN">1432-2242</idno><imprint><publisher>Springer-Verlag</publisher><pubPlace>Berlin/Heidelberg</pubPlace><date type="published" when="1996-05-01">1996-05-01</date><biblScope unit="volume">92</biblScope><biblScope unit="issue">6</biblScope><biblScope unit="page" from="660">660</biblScope><biblScope unit="page" to="665">665</biblScope></imprint><idno type="ISSN">0040-5752</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0040-5752</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Bulked-segregant analysis</term><term>Insect resistance</term><term>Marker-aided selection</term><term>Oryza sativa</term><term>RAPDs</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Abhaya</term><term>Amplification</term><term>Appl</term><term>Biotype</term><term>Dna</term><term>Fragment</term><term>Gall</term><term>Gall midge</term><term>Gall midge resistance gene</term><term>Gene</term><term>Genet</term><term>Genomic</term><term>Genomic dnas</term><term>Gin2</term><term>Gm4t</term><term>Homology</term><term>Insect pest</term><term>Marker</term><term>Midge</term><term>Mohan</term><term>Molecular markers</term><term>Nair</term><term>Nematode</term><term>Nucleic acids</term><term>Orseolia oryzae</term><term>Parental dnas</term><term>Polymerase</term><term>Polymerase chain reaction</term><term>Polymorphism</term><term>Primer</term><term>Proc natl acad</term><term>Rapd</term><term>Rapd fragment</term><term>Rapd fragments</term><term>Rapd markers</term><term>Rapds</term><term>Resistance genes</term><term>Resistant</term><term>Resistant bulk</term><term>Resistant parent</term><term>Rflp</term><term>Rflp markers</term><term>Rice gall midge</term><term>Sequence information</term><term>Simple sequence</term><term>Southern hybridisation</term><term>Specific primers</term><term>Susceptible</term><term>Susceptible bulk</term><term>Susceptible parent</term><term>Tanksley</term><term>Theor</term><term>Theor appl genet</term><term>Tulsi</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Rice DNAs from a gall midge resistant variety, ‘Abhaya’, a susceptible variety, ‘Tulsi’ and their F3 progeny were screened using 500 random primers in conjunction with bulked-segregant analysis in a polymerase chain reaction (PCR) with a view to detecting random amplified polymorphic DNAs (RAPDs) linked to the gene, Gm4t, which confers resistance to gall midge, a dipteran insect pest of rice. A total of 454 primers were able to produce a distinct amplification pattern, and 3695 bands/loci were amplified between the phenotypically different parents. Of these, 304 bands were polymorphic between the parents, with 19 being phenotypespecific. One of these primers, E20, amplified 2 bands, E20570 and E20583, which are tightly linked to resistance and susceptibility, respectively. These specific bands were cloned and sequenced, and a 94% sequence homology was found between the two fragments. Two specific 20-mer oligonucleotides were synthesized, based on the sequence information of E20583, for use in PCR amplification directly from genomic DNAs. These PCR primers were able to amplify phenotype-specific bands, a 583-bp fragment in susceptible F3 lines and a 570-bp fragment in resistant F3 lines that had been derived from a cross between the parents, indicating their potential and utility for marker-aided selection of the Gm4t gene in rice. Its use would facilitate the early and efficient selection of resistant genes in plant breeding programmes and even in those areas where the insect is not known to occur. These phenotype-specific bands are single-copy sequences and are being mapped to ascertain their chromosomal location in rice.</div></front></TEI><affiliations><list><country><li>Inde</li></country></list><tree><country name="Inde"><noRegion><name sortKey="Nair, S" sort="Nair, S" uniqKey="Nair S" first="S." last="Nair">S. Nair</name></noRegion><name sortKey="Kumar, A" sort="Kumar, A" uniqKey="Kumar A" first="A." last="Kumar">A. Kumar</name><name sortKey="Mohan, M" sort="Mohan, M" uniqKey="Mohan M" first="M." last="Mohan">M. Mohan</name><name sortKey="Srivastava, M N" sort="Srivastava, M N" uniqKey="Srivastava M" first="M. N." last="Srivastava">M. N. Srivastava</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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